March 15, 1984

Dr. Kwang W. Jeon

University of Tennessee Dept. of

Zoology

Knoxville, TN 37996

Dear Dr. Jeon:

On talking

with a number of cooperating physicians I find that

they need

guidance in how

to prepare the

knee effusion

samples. One

physician, for example, has refrigerated his two samples for two

weeks. Another

has used

antiamoebics

before submission.

Enclosed please note characteristics taken from Carter’s paper

on

Naeg

leria fowleri. If our search encompasses some of the physical

characteristics I’ve outlined, then some doctors will be destroying

the sample prior to your receipt.

Would you please send me a

sample

preparation and transportation proto-

col to mail to doctors?

Meanwhile

T

ony has collected a large

number

of papers from

19th onward

related to medical research with Hartmanella, Vahlkamfia, Naegleria,

et. al.

W

ould you like me to copy these

and

forward on t o you?

Cordially,

Perry A.

C

hapdelaine, Sr. C: Pybus,

Blount, Simoons